The Laboratory of Cell Research and Drug Delivery Systems

Laboratory description/carried out research

The Laboratory works on the development of new biomaterial technologies and the solutions for the  problems in the field of targeted therapy and regenerative medicine. Our research is focused on synthesis and characterization of implantable materials and biocompatible/haemocompatible surfaces, as well as delivery systems for drugs and other active substances.

Our approach  ischaracterised by the application of processes occuring directly in situ of the biomaterial with the maximum restriction of the organic solvents application.

Apart from biomaterials modification and analysis, the Laboratory conducts the study of the interactions between biomaterials and cells or tissues, with emphasis on in vitro cytotoxicity tests

 

Current research includes mainly:

– surface modifications of polymers and metals to obtain materials with high biocompatibility,

– creation of new inorganic-organic formulations having the ability to carry therapeutics based on selective targeting,

– modification of drug formulations to increase their bioavailability, biocompatibility and release time,

– in vitro mammalian cell cultures,

– cytotoxicity assays: XTT, MTT, MTS, LDH, etc.

– assessment of metabolism, differentiation, cell proliferation,

– microscopic observations of cells and material surfaces: optical microscopy, fluorescence microscopy, confocal microscopy

Equipment / devices

  • Nikon TS2 inverted phase contrast microscope with fluorescence and Fi3 camera
  • Diagnostic inverted microscope with phase contrast MW-50
  • Spectramax iD3 fluorescence and luminescence reader
  • Mictrotec manual rotary microtome with Medite paraffin embedding device
  • Infrastructure and apparatus for in vitro cell culture:
  • Thermo KS12 laminar flow chambers
  • incubators: Hera Cell with CO2, Biotek NB203 QS with CO2 with shaking
  • 3840 ELC-D Tuttnauer autoclave
  • MPW 352R and Pro Hospital LL centrifuges
  • dewar for storing cells in liquid nitrogen
  • ARCTIKO ULTF 220 freezer for freezing at -80°C
  • refrigerator with -18°C freezer
  • small equipment: shaker, thermometer, analytical and technical balances, vortexes, water bath, magnetic stirrers

Employees

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